Fig. 7

HMG20A bound to metastatic-specific enhancers to regulate ADRM1 and SLC12A7 expression. A/B, GSE120634 cohort was used for identification of enhancer regions (E1) around ADRM1 (A) and SLC12A7 (B) locus in HN120Met and HN120Pri cells. HN120Met is a metastatic OSCC cell line. HN120Pri is a primary OSCC cell line. C/D, BHY and HSC3 cells were transfected with si-HMG20A or si-NC. qRT-PCR and Western blotting were performed to measure mRNA (C) and protein (D) levels of HMG20A in si-HMG20A and si-NC groups. E/F, the status of H3K27ac modification in ADRM1 and SLC12A7 enhancer regions of BHY and HSC3 cells with or without HMG20A knockdown was examined by ChIP-qPCR. G/H, transcriptional levels of ADRM1 and SLC12A7 in BHY and HSC3 cells with or without HMG20A knockdown were detected by qRT-PCR. *P < 0.05, **P < 0.01, si-HMG20A group vs. si-NC group