Fig. 6

The downregulation of BACH1 impaired the odontoblastic differentiation of hDPSCs was HO-1-independent. a Transduced hDPSCs were cultured for 7 days. ALP activity was evaluated by ALP staining and ALP activity assay in the LV-NC, LV-shBACH1 and LV-shBACH1 + SnPP group. b Total protein level of odontogenic markers in LV-NC, LV-shBACH1 and LV-shBACH1+ SnPP hDPSCs on 14 days. Quantification of the relative protein expression of odontogenic markers via Image Pro Plus software. GAPDH was used as an internal control. Statistically significant compared to the control group. c hDPSCs were cultured for 21 days. Calcium nodule deposition was evaluated by ARS staining assay. Scale bar = 1 mm. SnPP, Tin-protoporphyrin IX. hDPSCS were cultured in OM or OM + SnPP (10 µM). Data were presented as the mean ± standard error of the mean (n = 3). Statistically significant compared to every other group. *P < 0.05, **P < 0.01, ***P < 0.001. The gels in b were cropped, full-length gels are presented in Additional file 1 Original Band of Western blot Analysis Fig. S4. The bands of DSPP, RUNX2 and GAPDH were from the same gel, while the band of DMP1 was from other gel