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Fig. 4 | BMC Oral Health

Fig. 4

From: Comprehensive analysis of M2 macrophage-derived exosomes facilitating osteogenic differentiation of human periodontal ligament stem cells

Fig. 4

Effect of M0-exo, M1-exo and M2-exo on osteogenic differentiation of hPDLSCs. A Fluorescence microscopic observation of exosome uptake by hPDLSCs after PKH67 labeling of M0-exo, M1-exo and M2-exo. Scale bar: 20 μm. B The effect of M0-exo, M1-exo and M2-exo on the formation of mineralized nodules was observed by ARS after 21 days of osteogenic induction. Original magnification: 100 × . C Spectrophotometric detection of absorbance of mineralized nodules using a spectrophotometer. Values are mean ± SD (n = 3 for each group). Compared to Induced group, *P < 0.05, **P < 0.01, ***P < 0.001, and ns means no significant difference

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