Table 1 Primers used in RT-qPCR
Target | Forward primer (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|
ALP | CTGAAGCCTCCGTGGAACAT | TGACCACAAAGACTCAGCTC |
OCN | CCCTTTCTCCTGTCCGGATG | GCTGAGCTCTAGGGGAGTCT |
β-actin | GGCTCTTTTCCAGCCTTCCT | AATGCCAGGGTACATGGTGG |
hsa-miR-6785-3p | GGAGGGCGTGGATGATGG | GGAAGGTGGGGCGATGTG |
hsa-miR-6507-3p | GCCGAGCAAAGUCCUUCC | GUGUGGUCAGUACCAUGC |
hsa-miR-4731-3p | GGTACAAGGTGCCTGTTGGA | CCTCAGGGTGAGGGATCTGA |
hsa-miR-4728-3p | AGGATGATGCACTGTTGGGG | CTTGACTCCCCAGTGAACCC |
hsa-miR-3614-5p | GTTCTGTCTTGGGCCACTTG | ACACCAAGATCTGAAGGCTAC |
hsa-miR-6780b-5p | CAGCCTGGGGAAGGCTTG | AAAGGAGACAAGGGAGAGGC |
hsa-miR-6085 | TGGGCCCAGCTTTACATAGT | CCTTCAGCACCTTTTGCAACC |
hsa-miR-4800-5p | GGAGTGGCCAGGAAGGAAG | CTGACAGGTAGGTGGACAGACG |
hsa-miR-4778-5p | TCGGCAGGCUGUAAAGG | CAGTGCAGGGTCCGAGGTA |
hsa-miR-1227-5p | GTGGTGGGCACTGCTGG | CTGGGGAAAAGGGTGGCA |
U6 | CCCTTCGGGGACATCCGATA | TTTGTGCGTGTCATCCTTGC |