Fig. 1

Light micrographs (LM) of H&E stained decalcified sections. A and D ×100 magnification. B, C, E, F ×400 magnification. A showing irregular trabeculation of the newly formed bone. The demarcation line between the native and the new bone (blue arrows). B higher magnification of the inset in the previous micrograph showing the extension of immature bone formation toward the defect center. C showing the discontinuous layer of osteoblasts (green arrows). D showing thick and well-organized bone trabeculae with primary osteon formation (arrowheads). E showing the interconnectivity and thickness of the trabeculae and their osteointegration with the native bone (blue arrows). F showing high activity of osteoblasts (green arrows)