Table 1 Primers and probes used in the real-time PCR assays
Bacteria | Target genes | Primers/probe sets | Amplicon size (bp) |
|---|---|---|---|
Parvimonas micra | 16S ribosomal RNA gene | 5′-GAGGAATACCGGTGGCGAAG-3’ 5′-GGCACCGAGATTTGACTCCC-3’ 5′-FAM-GGTACGAAAGCGTGGGGAGCA-BHQ1-3’ | 148 |
Staphylococcus aureus | Clumping factor A (clfA) gene | 5′-GCGCAAGTAACGAAAGCAAAA-3’ 5′-GATTTTGCGCCACACTCGTT-3’ 5′-FAM-TGCTGCACCTAAAACAGACGACACA-BHQ1-3’ | 132 |
Eubacterium nodatum | Hypothetical protein | 5′-TGCTTGCCGGTGACTTAGGA-3’ 5′-AAACCGGGCTCAACAACCAT-3’ 5′-Texas Red-TTGAGGAGCCGGTGACTTTGG-BHQ2-3’ | 130 |
Porphyromonas gingivalis | Hemagglutinin (phg) gene | 5′-ACACGGTGTATCGTGACGGC-3’ 5′-GCCGGCTGCGTACTTAACCT-3’ 5′-HEX-CGACCTACCGCGATGCAGGA-BHQ1-3’ | 119 |
Treponema denticola | Oligopeptidase B (opdB) gene | 5′-AGAAAGGCTTTGGGCGACAG-3’ 5′-GCTGGAGCCGTAGCTTCCAT-3’ 5′-Cy5-CGGGTCCTCACCCGCTCTTC-BHQ2-3’ | 127 |
Fusobacterium nucleatum | 16S ribosomal RNA gene | 5′-GGCTGTCGTCAGCTCGTGTC-3’ 5′-CTCATCGCAGGCAGTATCGC-3’ 5′-FAM-AACGAGCGCAACCCCTTTCG-BHQ1-3’ | 114 |
Prevotella intermedia | hemagglutinin (phg) gene | 5′-CACACGCTGGCGAAACCTAC-3’ 5′-CACGTGGCGTTGCTTCTTTC-3’ 5′-HEX-CCGAAGATGCGCCGTTGAAC-BHQ1-3’ | 143 |
Prevotella nigrescens | Gyrase subunit B (gyrB) gene | 5′-AGCAAGCTGTAGGCGAGGCT-3’ 5′-GCTGAACACTTTCGCGTGCT-3’ 5′-Texas Red-GCTCGTATTGCAGCCCGCAA-BHQ2-3’ | 132 |
Eikenella corrodens | Proline iminopeptidase (pip) gene | 5′-GCCAACTGCTGCTGGAAGTG-3’ 5′-GCCGCTGATTTCGGAGAGTT-3’ 5′-HEX- ACAGCCATCGGCACAGGCAT-BHQ1–3’ | 110 |
Campylobacter rectus | GroEL gene | 5′-AAATTTAAGCGGCGACGAGG-3’ 5′-TCCTTGCTCACGCTTACGGA-3’ 5′-HEX-GGCTTTGACGCGGGCGTAGT-BHQ1-3’ | 132 |