Fig. 4

Knockdown of METTL3 inhibited the dentinogenesis differentiation of DPSCs. A METTL3-kncokdown lentiviral were used to knockdown METTL3 (shMETTL3-a, shMETTL3-b) in DPSCs. The knockdown efficiency of METTL3 gene expression in DPSCs, detected by real‐time RT‐PCR (n = 3). B Alizarin red S staining on 14 d, and C)ALP staining on 7 d of DPSCs cultured in OM (n = 3), C The quantitative analysis and staining of ALP activity (n = 3). DThe knockdown efficiency of METTL3 gene expression detected by western blotting (n = 3). E Expression levels of dentinogenesis differentiation-related genes, OCN, RUNX2, OSX, DSPP, were measured by real‐time RT‐PCR on 0, 7 and 14 days after mineralization induction (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001