Table 1 Primers used for real-time quantitative PCR
Gene | Forwards primer | Reverse primer |
|---|---|---|
IL-4 | CCAACTGCTTCCCCCTCTG | TCTGTTACGGTCAACTCGGTG |
IL-6 | ACTCACCTCTTCAGAACGAATTG | CCATCTTTGGAAGGTTCAGGTTG |
IL-10 | GACTTTAAGGGTTACCTGGGTTG | TCACATGCGCCTTGATGTCTG |
IL-1β | ATGATGGCTTATTACAGTGGCAA | GTCGGAGATTCGTAGCTGGA |
TNF-α | CGTGGAGCTGGCCGAGGAG | AGGAAGGAGAAGAGGCTGAGGAAC |
OCN | AGCAAAGGTGCAGCCTTTGT | GCGCCTGGGTCTCTTCACT |
ALP | GTTGACACCTGGAAGAGCTT | GTTCCTGTTCAGCTCGTACTG |
RUNX2 | TGGTTACTGTCATGGCGGGTA | TCTCAGATCGTTGAACCTTGCTA |
GAPDH | GGAGCGAGATCCCTCCAAAAT | GGCTGTTGTCATACTTCTCATGG |