Fig. 1
From: The role of EMILIN-1 in the osteo/odontogenic differentiation of dental pulp stem cells
The isolation and characterization of hDPSCs. A Morphology of the human pulp stem cells (hDPSCs) colonies formed after 3 d (a) and 5 d (b) of pulp tissue isolation and culture. The fifth generation hDPSCs were cultured for 24 h (c), with cells arranged in bundles or spirals, and after 48 h (d), cells proliferated reached 80% confluence. B Flow cytometry to identify mesenchymal stem cell properties of hDPSCs. C Colony forming unit of hDPSCs. D Alkaline phosphatase (ALP) staining. E Alizarin red S (ARS) staining. Scale bars = 250 μm