Fig. 6

rhEMILIN-1 coating stimulated osteo/odontogenic differentiation of hDPSCs. A CCK8 assay showed that rhEMILIN-1 treatment significantly increased the proliferation of hDPSCs. B ALP staining (7 days) showed that rhEMILIN-1 decreased ALP activity in hDPSCs. Scale bars = 250 μm. C ARS staining (21 days) showed that rhEMILIN-1 treatment significantly increased the mineralization capacity of hDPSCs (a). Mineralized nodules was quantified (b). Scale bars = 250 μm. D qPCR detected that rhEMILIN-1 treatment increased the relative mRNA expression of osteo/odonto-specific genes at osteo/odontogenesis day 7 (a) and osteo/odontogenesis day 21(b). E Western blot detected that rhEMILIN-1 treatment increased the expression of osteo/odonto-specific proteins in hDPSCs at early and late stages of induction (a). Grayscale analysis of protein bands (b). Values are presented as mean ± standard deviation (SD). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. The blots in Ea were cropped