Table 1 Species-specific primers for real-time qPCR assay
Microorganisms | Primers (5’-3’) | Amplicon size | Reference |
|---|---|---|---|
S. mutans | GCC TAC AGC TCA GAG ATG CTA TTCT GCC ATA CAC CAC TCA TGA ATT GA | 114 | Childers [28] |
S. sobrinus | GGA CTT GCT CCA GTG TTA CTA ATG AG CCG CTA TCA GGC AGG TTA CC | 88 | Price [13] |
Bifidobacterium spp. | GGG TGG TAA TGC CGG ATG CCA CCG TTA CAC CGG GAA | 498 | Mantzourani [29] |
Lactobacillus spp. | TGG AAA CAG ATG CTA ATA CCG CGT CCA TTG TGG TAG ATT CCC T | 223 | Bizhang [20] |
A. naeslundii | CTCCTACGGGAGGCAGCAG CACCCACAAACGAGGCAG | 135 | Dalwai [17] |