Fig. 3

Stevioside exposure reduced the number of osteoclasts, the RNA expression of inflammatory cytokines, and P. gingivalis in periodontal tissue in mice. A Tartrate-resistant acid phosphatase (TRAP) staining (arrows). The osteoclast number of the P+S group was lower than that in P and P+G groups (P＜0.05). B CTSK staining (arrows) after modeling. The CTSK-positive number of cells in the P+S group was lower than that in P and P+G groups (P < 0.05). C The relative quantification of qPCR of TNF-a in gingiva tissues. D The relative quantification of qPCR of IL -1β in gingiva tissues. E The relative quantification of qPCR of IL-6 in gingiva tissues. F The relative quantification of qPCR of the 16S rRNA gene of P. gingivalis in gingiva tissues. *P < 0.05. **P < 0.01. ***P < 0.001. The scale bars in (A) and (B) were 500 μm and 50 μm