Fig. 2

RTG and CT analysis and immunohistochemistry of the first collected oral sample of our patient. First X-ray of patient with ameloblastoma, where is retained wisdom tooth and loss of bone at the right side of mandible (A). 3D reconstruction of mandible from CT scans. There is a large lesion with bone resorption primary in the right side of the mandible extending into the region of the mandibular ramus (B). Epithelial cords of tumourous components with columnar cells exhibiting reverse polarity; Haematoxylin–Eosin staining, magnification 200x (C). Diffuse membranous positivity of pan-cytokeratin in tumourous cells; immunohistochemistry: CK AE1/AE3 (pan-cytokeratin (1:1, cat. no. 961, Abcam, UK), magnification 200x (D). Minimal nuclear immunoreactivity of p53 in tumour cells; immunohistochemistry: p53, magnification 200x (E). Slight nuclear positivity in Ki67 staining displays a weak proliferation activity of tumorous tissue; immunohistochemistry: Ki67 (1:200, cat. no. 275-R-16, Cell Marque, USA), magnification 200x (F). 3,3′-diaminobenzidine (DAB, cat. No. K3468, DAKO, Agilent Technologies, USA) was used to detect positive cells and Haematoxylin was applied to counterstain the nuclei