Fig. 5
From: Metastasising ameloblastoma or ameloblastic carcinoma? A case report with mutation analyses
RNA expression analysis of Wnt related genes and β-catenin immunofluorescent detection. Expression analysis of genes related to Wnt signalling pathway. Ameloblastoma tumour tissue sample was compared to three healthy gingival tissues (represented by three data points for each gene). Values for gene expression changes were calculated using 2-ΔΔCT method and subsequently log2 transformed (A). In the control oral epithelial cells, β-catenin signal is membranous, and it is regularly distributed through cell surfaces (B). In ameloblastic carcinoma, there is a signal loss in the tumorous cells and the expression pattern is discontinuous and stippled (C). Immunofluorescence: β-catenin (6B3) (beta-catenin (1:100, cat. no. 9582, Cell Signalling Technology, USA), the nuclei were counterstained by DRAQ5™ (cat. no. 62251, Thermo Fisher Scientific)