Table 1 Scoring categories for osteogenic, chondrogenic and adipogenic stimulated pellets in the monolayer culture system
From: TNF-α, IL-1B and IL-6 affect the differentiation ability of dental pulp stem cells
Scoring categories | Scores | |
|---|---|---|
A. Uniformity and darkness | ||
No stain | 0 | |
Weak staining of poorly formed matrix | 1 | |
Moderately even staining | 2 | |
Even dark stain | 3 | |
B.Distance between cells/Amount of matrix accumulated | ||
Osteogenic | No osteogenic colonies/No calcium deposit | 0 |
No osteogenic colonies/Weak staining of calcium deposits | 1 | |
Osteogenic colonies/Waek staining of calcium deposits | 2 | |
Osteogenic colonies/High amount of calcium deposits | 3 | |
Chondrogenic | Low cell densities with no matrix between cells | 0 |
Low cell densities with little matrix between cells | 1 | |
Moderate cell density with matrix | 2 | |
High cell density with moderate distance between cells | 3 | |
Adipogenic | No adipocytes/No oil droplets | 0 |
No adipocytes/Weak staining of oil droplets | 1 | |
Adipocytes with weak staining of oil droplets | 2 | |
Adipocytes with high amount of oil droplets | 3 | |
C. Cell morphologies represented | ||
Osteogenic | Condensed/necrotic/pycnotic bodies | 0 |
Spindle/fibrous | 1 | |
Mixed spindle/fibrous with calcium deposits | 2 | |
Majority calcium deposits/osteogenic | 3 | |
Chondrogenic | Condensed/necrotic/pycnotic bodies | 0 |
Spindle/fibrous | 1 | |
Mixed spindle/fibrous with cartilage forming | 2 | |
Majority cartilage forming/chondrogenic | 3 | |
Adipogenic | Condensed/necrotic/pycnotic bodies | 0 |
Spindle/fibrous | 1 | |
Mixed spindle/fibrous with oil droplets | 2 | |
Majority oil droplets/adipogenic | 3 | |