Fig. 2

Periodontitis induced ERS and decreased the expression of adiponectin in rat visceral adipocytes. (A) The mRNA levels of GRP78, IRE1α and PERK in the periodontitis group were significantly higher than those in the control group, but ATF6 did not change. (B) Western blotting image of GRP78, IRE1α, p-IRE1α, PERK, p-PERK and ATF6 expression. (C) GRP78 and p-IRE1α/IRE1α expression was significantly higher than that in the control group, but p-PERK/PERK and ATF6 expression was not altered. (D) The mRNA level of adiponectin in the periodontitis group was lower than that in the control group. (E) Western blotting image of adiponectin expression. (F) Adiponectin expression was lower than that in the control group. The samples derive from the same experiment and that blots were processed in parallel. The full-length blots are presented in Supplementary File 1. Fig. 1S. GRP78 = glucose-regulated protein 78; IRE1α = inositol-requiring protein 1α; p-IRE1α = phosphorylation levels of IRE1α; PERK = double-stranded RNA-dependent protein kinase (PKR)-like ER kinase; p-PERK = phosphorylation levels of PERK; ATF6 = activating transcription factor 6. Data are presented as the mean ± SD. *P < 0.05, **P < 0.01 vs. the control group