Fig. 3

P.g-LPS induced ERS and downregulated adiponectin expression in visceral adipocytes in vitro. (A) Optical microscope images show the culture of visceral adipocytes and Oil Red O staining for identifying adipocytes (scale bar, 200 μm). (B) CCK8 analysis of P.g-LPS concentration on the proliferation of visceral adipocytes. (C) Western blotting image of GRP78, IRE1α, and p-IRE1α expression in visceral adipocytes treated with 100 ng/mL P.g-LPS for 0, 4, and 8 h. (D) GRP78 and p-IRE1α/IRE1α expression was upregulated in a time-dependent manner. (E) Western blotting image of adiponectin expression in visceral adipocytes treated with 100 ng/mL P.g-LPS for 0, 4, and 8 h. (F) Adiponectin expression significantly decreased in a time-dependent manner. The samples derive from the same experiment and that blots were processed in parallel. The full-length blots are presented in Supplementary File 1. Fig. 2S. Data are presented as the mean ± SD. *P < 0.05, **P < 0.01 vs. the 0 h group, #P < 0.05, ##P < 0.01, ###P < 0.001 vs. the 4 h group