Fig. 4

Effect of IRE1α overexpression and inhibition on adiponectin expression in visceral adipocytes. (A) The mRNA level of adiponectin in the pLVX-IRE1α group was lower than that in the pLVX-NC group. The adiponectin mRNA level in the pLVX-IRE1α + P.g-LPS group was significantly decreased compared to that in the pLVX-NC + P.g-LPS group. (B) Western blotting image of adiponectin expression. (C) Adiponectin expression in the pLVX-IRE1α group was lower than that in the pLVX-NC group. The expression of adiponectin in the pLVX-IRE1α + P.g-LPS group was decreased compared to that in the pLVX-NC + P.g-LPS group. (D) Compared with the pLVX-NC group, the mRNA level of adiponectin was increased in the pLVX-IRE1α-RNAi group. The adiponectin mRNA level in the pLVX-IRE1α-RNAi + P.g-LPS group was higher than that in the pLVX-NC + P.g-LPS group. (E) Western blotting image of adiponectin expression. (F) Adiponectin expression was increased in the pLVX-IRE1α-RNAi group compared with the pLVX-NC group. The expression of adiponectin in the pLVX-IRE1α-RNAi + P.g-LPS group was higher than that in the pLVX-NC + P.g-LPS group. The samples derive from the same experiment and that blots were processed in parallel. The full-length blots are presented in Supplementary File. 1 Fig. 3S. Data are presented as the mean ± SD. *P < 0.05, **P < 0.01 vs. the pLVX-NC group, #P < 0.05, ##P < 0.01 vs. the pLVX-NC + Pg-LPS group