Table 1 List of primer sequences used in Real-time PCR
Gene | Primer sequence |
|---|---|
GAPDH(human) | Forward:CAAGGCTGTGGGCAAGGTCATC Reverse:GTGTCGCTGTTGAAGTCAGAGGAG |
GAPDH(mouse) | Forward:TGAAGGTCGGTGTGAACGGATTTG Reverse:TCGCTCCTGGAAGATGGTGATGG |
VEGF | Forward:GCCTTGCCTTGCTGCTCTACC Reverse:CTTCGTGATGATTCTGCCCTCCTC |
HIF-1α | Forward:ACCGCTGAAACGCCAAAG Reverse:TCCATCGGAAGGACTAGGTGTCT |
COL-1α1 | Forward:GACAGGCGAACAAGGTGACAGAG Reverse:CAGGAGAACCAGGAGAACCAGGAG |
OCN | Forward:CAAGCAGGAGGGCAATAAGGTAGTG Reverse:CGGTCTTCAAGCCATACTGGTCTG |
RUNX2 | Forward:GCAGCACTCCATATCTCTACT Reverse:TTCCGTCAGCGTCAACAC |