Fig. 5

Immunofluorescence staining of OPN. Fourteen days after transfection, the OPN expression was detected by immunofluorescence to indicate the osteogenic differentiation of ASCs (1–4), EGFP/ASCs (5–8), and BMP-2/ASCs (9 – l2). The nuclei are stained blue by DAPI; the cytoskeleton is green as stained by phalloidin; OPN secretions are listed in the third column. The highest secretion is in the BMP-2/ASCs, while limited expression in the ASCs and EGFP/ASCs; The fourth column shows the merged images of the nuclei, skeleton, and target protein, OPN