Fig. 3
From: M2 macrophages-derived exosomes regulate osteoclast differentiation by the CSF2/TNF-α axis
CSF2 is highly expressed in M2-exos and is required for osteoclastogenesis. (A) CSF2 mRNA expression in M2 macrophages. (B) CSF2 mRNA expression in RANKL-treated Raw264.7 cells. (C) Protein levels of CSF2 in M2 macrophages and RANKL-induced Raw264.7 cells. (D) The efficiency following si-NC, si-CSF2#1, and si-CSF2#2 transfection. (E) Osteoclast differentiation was analyzed using the TRAP staining assay. Osteoclast markers (F) TRAP, (G) NFATC1, and (H) Cathepsin K were detected by qPCR. **P < 0.01 vs. the control or si-NC groups. ##P < 0.01 vs. the RANKL + M2-exos + si-NC group