Table 1 Primer sequences used in real-time PCR assay

GAPDH

5′- CAGGAGGCATTGCTGATGAT − 3’

5′- GAAGGCTGGGGCTCATTT − 3’

RUNX2

5′- CACAAGTGCGGTGCAAACTT − 3’

5′- TGCTTGCAGCCTTAAATGACT − 3”

OSX

5′- CTCCCTCCTGGCCATTCTGG − 3’

5′- GGAAGCCGGAGTGCAGGTAT − 3’

ALP

5′- CTGGACCTCGTTGACACCTG − 3’

5′- TCCGTCACGTTGTTCCTGTT − 3’

COL-1

5′- GAGGGCCAAGACGAAGACATC − 3’

5′- CAGATCACGTCATCGCACAAC − 3’

OCN

5′- CTCACACTCCTCGCCCTAT −3’

5′- TCTCTTCACTACCTCGCTGC −3’