Fig. 1
From: Oral cancer cell to endothelial cell communication via exosomal miR-21/RMND5A pathway
RMND5A inhibits the proliferation, migration and tube formation of HUVECs. (a, b) Overexpression of Flag-tag RMND5A in HUVECs via lentiviral infection. Real-time PCR (a) and WB (b) data proves the elevated expression of RMND5A in HUVECs. (c) MTT assay shows ECGs dependent growth of HUVECs. By comparison with vector control group, overexpression of RMND5A significantly inhibits the proliferation of HUVECs under the condition of ECGs. (d) Would healing assay shows that overexpression of RMND5A significantly inhibited the migration ability of HUVECs. (e) Overexpression of RMND5A reduces the tube formation ability of HUVECs evidenced by decreased capillary-like structures. Data is expressed as mean ± SD. ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001