Fig. 3
From: Oral cancer cell to endothelial cell communication via exosomal miR-21/RMND5A pathway
OSCC cell-derived exosomes inhibit RMND5A gene expression of HUVECs. (a, b) CAL27 (a) and SCC25 (b) cell culture supernatant (condition media, CM) dose-dependently decreases RMND5A gene expression in HUVECs after 24-h treatment, but does not change RanBPM gene expression. (c) The exosomes are purified from the condition media by differential centrifugation and confirmed by Nanosight. (d, e) The exosomes from CAL27 (d) and SCC25 (e) cells, but not exosome-depleted CM, significantly decrease RMND5A gene expression in HUVECs after 24-h treatment. Data is expressed as mean ± SD. ns, not significant; *P < 0.05; **P < 0.01