Fig. 1

NRG-1 promotes proliferation and migration of DPSCs. (A(a)) DPSCs were observed to exhibit a long fusiform and swirling arrangement under the microscope. (A(b)) Osteogenic differentiation induced the formation of mineralization nodules. (A(c)) Lipid differentiation resulted in the formation of lipid droplets. (B, C) The transfection efficiency of NRG-1 was assessed through Western Blot detection and subsequent statistical analysis. (D) The transfection efficiency of NRG-1 was assessed using RT-PCR. (E) The impact of NRG-1 on the migratory capacity of DPSCs at 0, 24, and 48 h was evaluated using the scratch method. (F, G) The width of the scratch was subjected to statistical analysis. (H) The proliferation ability of DPSCs was examined at 1, 3, and 5 days using the CCK-8 method, in order to determine the effect of NRG-1. Statistical significance was determined using the following notation: (ns (P > 0.05), ** (P < 0.001), *** (P < 0.0001), **** (P < 0.00001)). The blots in B were cropped