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Fig. 2 | BMC Oral Health

Fig. 2

From: N-acetylcysteine as a novel methacrylate-based resin cement component: effect on cell apoptosis and genotoxicity in human gingival fibroblasts

Fig. 2

DNA-DSBs induced by Premier® and protective effects of incorporated NAC. a Representative images of immunofluorescent staining for H2AX phosphorylation (orange) in HGFs, after exposure to different substances compared to control cells. Sybr green (green) is a marker for DNA and stains the whole nucleus of the cell. Nucleus of HGFs with typical γ-H2AX-specific foci can be observed in Premier® and Premier® + NAC(0.1 wt.%)treated groups. b Average of induced γ-H2AX DSBs-foci/cell in HGFs treated with different samples. Data are expressed as mean ± SD (n = 3); p < 0.01

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