Using formalin fixed paraffin embedded tissue to characterize the microbiota in p16-positive and p16-negative tongue squamous cell carcinoma: a pilot study

Table 1 Primers used for 16 S rRNA gene sequencing analysis

Region	Name	F/R	Sequence
V1–V2	27 F/338R [25]	F	5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-3′
V1–V2	27 F/338R [25]	R	5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-3′
V3-V4	338 F/806R [26]	F	5′-ACTCCTACGGGAGGCAGCAG-3′
V3-V4	338 F/806R [26]	R	5′-GGACTACHVGGGTWTCTAAT-3′
V4	515 F/806R [23]	F	5′‑GTGCCAGCMGCCGCGG-3′
V4	515 F/806R [23]	R	5′GGACTACHVGGGTWTCTAAT-3′

ISSN: 1472-6831