Table 2 DNA quality assessment by different primers
Primer | The number of samples | PCR pre-experiment | Â | PCR formal experiments | Â | Success rate of sequencing library construction | ||||
|---|---|---|---|---|---|---|---|---|---|---|
A | B | C |  | A | B | C |  | A + B/ total number of cases | ||
515Â F/806R | 60 | 0 | 12(6 p16+/6 p16-) | 48 | Â | 8 | 2 | 2 | Â | 16.67% |
27Â F/338R | 60 | 0 | 15(9 p16+/6 p16-) | 45 | Â | 10 | 1 | 4 | Â | 18.33% |
338Â F/806R | 60 | 0 | 5(3 p16+/2 p16-) | 55 | Â | 5 | 0 | 0 | Â | 8.33% |