Newly discovered clouting interplay between matrix metalloproteinases structures and novel quaternary Ammonium K21: computational and in-vivo testing

Table 2 Results for main study plate incorporation assay

K21 mg per plate	Colony Counts			Mean ± SD	Fold	p value	Mean ± SD of Transformed	Lawn	Precipitate
TA98, -S9
0	31	32	33	32 ± 1	1.0		5.66 ± 0.09	NL	NP
0.0016	31	36	38	35 ± 4	1.1		5.91 ± 0.31	NL	NP
0.0051	32	34	35	34 ± 2	1.1		5.80 ± 0.13	NL	NP
0.016	24	28	38	30 ± 7	0.9		5.45 ± 0.65	NL	NP
0.051	29	30	31	30 ± 1	0.9		5.48 ± 0.09	NL	NP
0.16	17	30	33	27 ± 9	0.8		5.11 ± 0.87	NL	NP
0.50	12	14	16	14 ± 2	0.4		3.74 ± 0.27	ER	SP
2-NF, 5 µg	3212	3605	3809	3542 ± 303	110.7	< 0.0001	59.48 ± 2.57	NL	NP
TA98, + S9
0	38	40	41	40 ± 2	1.0		6.30 ± 01.2	NL	NP
0.0051	41	44	45	43 ± 2	1.1		6.58 ± 0.16	NL	NP
0.016	43	43	44	43 ± 1	1.1		6.58 ± 0.04	NL	NP
0.051	37	42	45	41 ± 4	1.0		6.42 ± 0.32	NL	NP
0.16	45	46	47	46 ± 1	1.2		6.78 ± 0.07	NL	NP
0.50	36	39	50	42 ± 7	1.1		6.44 ± 0.56	NL	SP
1.6	24	28	31	28 ± 4	0.7		5.25 ± 0.34	*	MP
B[a]P, 5 μg	520	553	603	559 ± 42	14.0	< 0.0001	23.63 ± 0.88	NL	NL
TA100, -S9
0	131	135	159	142 ± 15	1.0		11.89 ± 0.63	NL	NP
0.0016	119	139	154	137 ± 18	1.0		11.70 ± 0.75	NL	NP
0.0051	139	145	148	144 ± 5	1.0		12.00 ± 0.19	NL	NP
0.016	139	140	147	142 ± 4	1.0		11.92 ± 0.18	NL	NP
0.051	125	151	155	144 ± 16	1.0		11.97 ± 0.69	NL	NP
0.16	118	119	122	120 ± 2	0.8		10.94 ± 0.09	NL	NP
0.50	17	24	38	26 ± 11	0.2		5.06 ± 1.03	ER	SP
Na Az, 5 μg	1986	1849	1857	1897 ± 77	13.4	< 0.0001	43.55 ± 0.88	NL	NP
TA100, + S9
0	153	153	165	157 ± 7	1.0		12.53 ± 0.27	NL	NP
0.0051	134	161	174	156 ± 20	1.0		12.49 ± 0.83	NL	NP
0.016	136	152	156	148 ± 11	0.9		12.16 ± 0.44	NL	NP
0.051	150	178	178	169 ± 16	1.1		12.98 ± 0.63	NL	NP
0.16	133	148	160	147 ± 14	0.9		12.12 ± 0.56	NL	NP
0.50	139	139	159	146 ± 12	0.9		12.06 ± 0.47	NL	SP
1.6	87	104	106	99 ± 10	0.6		9.94 ± 0.53	*	MP
B[a]P, 5 μg	1508	1500	1403	1470 ± 58	9.4	< 0.0001	38.34 ± 0.77	NL	NP

B[a]P Benzo[a]pyrene, NaAz Sodium azide, 2-NF 2-Nitrofluorene, Transformed Square root of colony counts, Fold Mean colony counts ÷ Mean colony counts of negative control, NL Normal, SR Slight Reduction, MR Moderate Reduction, ER Extreme Reduction, AB: Absence, NP None, SP Slight, MP Moderate, EP Extreme
p-values calculated if Fold ≥ 3.0 (TA1535 and
TA1537) or ≥ 2.0 (TA98, TA100, and
WP2 uvrA)
Background Lawn Toxicity:
^*: Lawn not scorable due to precipitate
Test Item Precipitate:
Colony Counts Mean ± SD Fold Lawn Precipitate
Mean ± SD

ISSN: 1472-6831