Table 2 Order, amounts, weighing containers, purities, and formula weights of reagents for preparing 1000 ml of SBF

1

NaCl

8.035 g

Weighing Paper

99.5

58.4430

2

NaHCO₃

0.355 g

Weighing Paper

99.5

84.0068

3

KCl

0.225 g

Weighing Bottle

99.5

74.5515

4

K₂HPO₄.3H₂O

0.231 g

Weighing Bottle

99.0

228.2220

5

MgCl₂.6H₂O

0.311 g

Weighing Bottle

98.0

203.3034

6

1.0 M-HCl

39 ml

Graduated Cylinder

-

-

7

CaCl₂

0.292 g

Weighing Paper

95.0

110.9848

8

Na₂SO₄

0.072 g

Weighing Bottle

99.0

142.0428

9

Tris

6.118 g

Weighing Bottle

99.0

121.1356

10

1.0 M-HCl

0–5 ml

Syringe

-

-