- Research article
- Open Access
The effect of intracanal medicaments used in Endodontics on the dislocation resistance of two calcium silicate-based filling materials
BMC Oral Health volume 20, Article number: 57 (2020)
Intracanal medicaments can be used in various endodontic conditions including multiple visit endodontics after trauma or in regenerative endodontics. These medicaments should be removed from the root canal before the placement of the filling or repair material. The aim of the present study was to evaluate the effect of prior calcium hydroxide (Ca(OH)2) and modified triple antibiotic paste (mTAP) placement on the push-out bond strength of TotalFill BC fast set putty (BC fast set putty) to root dentin when compared to mineral trioxide aggregate (MTA).
The root canals of 45 extracted mandibular premolars were prepared to a standardized internal diameter (1.5 mm). The specimens were randomly assigned to 3 groups according to the intracanal medicament used: mTAP (a mixture of metronidazole, ciprofloxacin, and cefaclor), Ca(OH)2, and no intracanal medicament. After 1 week, the medicaments were removed, and the middle third of the roots were cut into two transverse sections (2.0 ± 0.05) (n = 90 slices). Thereafter, the specimens were divided into two subgroups (n = 45 each): MTA or BC putty. After 1 week, the push-out test was performed and failure mode was evaluated. The data were statistically analyzed using two-way ANOVA and Tukey’s post hoc.
The application of the intracanal medicament did not significantly affect the bond strength of BC putty (p > .05). For MTA, the prior application of Ca(OH)2 or mTAP significantly decreased the dislocation resistance (p < .05). Specimens in the MTA subgroups showed an almost equal number of cohesive and mixed types of failure while the majority of the specimens in the BC putty subgroups revealed the cohesive type.
Ca(OH)2 and mTAP promoted lower bond strength of MTA to root dentin compared to the control group. However, the BC fast set putty bond strength to dentin was not affected by prior medication with Ca(OH)2 or mTAP.
The role of microorganisms as the primary cause of apical periodontitis is well documented in the literature [1, 2]. Therefore, endodontic treatment procedures are directed toward the elimination of microorganisms from the root canal system. Bacterial elimination is performed using chemomechanical debridement. However, this use of instrumentation and irrigation does not render the root canal free of bacteria . The use of an interappointment medicament has been shown to significantly improve disinfection after chemomechanical procedures . Calcium hydroxide [Ca(OH)2] is a commonly used intracanal medicament. It possesses several advantages such as tissue dissolving ability  and antibacterial properties . Triple antibiotic paste (TAP) is another example of an intracanal medicament. It is composed of ciprofloxacin, metronidazole, and minocycline . This mixture has been used for disinfection of the root canal system during root canal treatment and pulpal regeneration [8, 9]. The main drawback of TAP is tooth discoloration caused by minocycline . Therefore, cefaclor has replaced minocycline in this paste and is described as modified triple antibiotic paste (mTAP) .
Calcium silicate-based cements are commonly used in endodontics for different clinical procedures including pulpal regeneration, apexification, and perforation repair . Mineral trioxide aggregate (MTA), a calcium silicate-based cement, has been widely used in endodontics since its development in the 1990s . MTA has several desirable properties such as biocompatibility  and bioactivity . The main disadvantages of MTA are the long setting time , difficulty in handling , and discoloration potential . TotalFill BC (FKG, Dentaire, La Chaux-de-Fonds, Switzerland; marketed as EndoSequence BC from Brasseler USA in North America) is a newer calcium silicate-based cement that was developed to overcome the disadvantages of MTA. It is a biocompatible  premixed cement that sets in the presence of water and has shorter setting time than MTA . It is also reported that BC is a bioactive cement  with similar compressive strength to MTA . Furthermore, it does not induce discoloration in the tooth structure .
After canal disinfection and the medication period, the intracanal medicament should be removed from the root canal before the placement of the filling or repair material. One of the requirements of calcium silicate cements is resistance to dislodgement when exposed to occlusal or operative procedures . Previous studies reported that Ca(OH)2, TAP and mTAP do not affect the dislocation resistance of MTA to root dentin [23, 24]. However, Turk et al.  found that the application of TAP for longer than 2 weeks or Ca(OH)2 for longer than 4 weeks has a negative effect on MTA-dentin bond strength. To the best of our knowledge, no studies have evaluated the effects of intracanal medicaments on the dislocation resistance of BC cement to root canal dentin. Therefore, the purpose of the present study was to evaluate the effect of prior Ca(OH)2 and mTAP placement on the push-out bond strength of TotalFill BC root repair material fast set putty to root dentin compared to those of MTA. The null hypothesis was that intracanal medicaments would not affect the bond strength of TotalFill BC root repair material and MTA.
This research was approved by the Institutional Review Board of King Saud University in Riyadh, Saudi Arabia (E-18-3078), and the College of Dentistry Research Center (FR0446) of King Saud University in Riyadh, Saudi Arabia. Sample size calculation was performed using G*Power 126.96.36.199 software at alpha error probability of 0.05 with effect size of 0.43 and power of 0.88. The power analysis showed that a total of 90 specimens (15 per group) were required.
Specimens were prepared as described previously by Nagas et al.  with some modifications. Forty-five extracted single-rooted human mandibular premolars were selected. Teeth had been extracted for reasons unrelated to this study. The teeth were examined under dental operating microscope (Zeiss, Oberkochen, Germany) to exclude any teeth with root caries, cracks, or fractures. Teeth with one noncalcified canal, as confirmed by radiographs taken from buccolingual and mesiodistal views, were selected. Premolars with canals of more than a 20° curvature  were excluded. The teeth were stored in saline until used.
The teeth were embedded vertically in epoxy resin (Vertex Orthoplast; Vertex-Dental, Zeist, Netherlands). Specimens were sectioned apically 12 mm and coronally 1 mm below the cementoenamel junction using an Isomet low speed saw (Isomet; Buehler Ltd., Lake Blu, NY, USA) with continuous water irrigation. The working length was determined by placing a size 10 K-file (Maillefer, Ballaigues, Switzerland) in the canal until it was visible at the apical foramen. The glide path was established by hand filing with 15 and 20 K-file sizes to full working length. Instrumentation was completed with ProTaper rotary instruments (Dentsply Maillefer, Ballaigues, Switzerland) to the F3 file (tip size 30 and 0.09 taper) at 300 rpm. To obtain a standard parallel internal diameter of 1.5 mm, Peeso reamers (Maillefer, Ballaigues, Switzerland) between #1 and #5 were introduced in the root canals, and a #5 Peeso reamer was allowed to protrude 1 mm beyond the apex. Each instrument was replaced after preparing three canals or if any sign of deformation was observed. The root canals were irrigated using 2 mL 2.5% sodium hypochlorite (NaOCl) after each instrument. After completion of the instrumentation process, the smear layer was removed with 10 mL 17% ethylenediaminetetraacetic acid (EDTA) (Pulpdent, Watertown, USA) applied over a period of 1 min followed by 10 mL of 2.5% NaOCl. The canals were then flushed with 20 mL sterile saline to remove the residual irrigant and dried with sterile paper points (Dentsply Maillefer, Tulsa, Oklahoma, USA). A single operator prepared the root canals under dental operating microscope.
The specimens were randomly assigned to 3 groups (n = 15 each) with respect to the intracanal medicament used: mTAP, Ca(OH)2, and the control group. mTAP was prepared by mixing equal portions of metronidazole (Eczacibasıbası, Istanbul, Turkey), ciprofloxacin (Bayer, Leverkusen, Germany), and cefaclor (Sanovel, Istanbul, Turkey) with sterile distilled water (powder/liquid ratio of 3:1) . UltraCal XS (Ultradent, South Jordan, UT) was used for the Ca(OH)2 group. No intracanal medicament was placed in the control group. The medicament was delivered into the canals using a lentulo spiral (Dentsply Maillefer, Ballaigues, Switzerland) in a slow-speed handpiece and tamped down in the canal space using the blunt ends of sterile paper points. The apical root canal and coronal orifices were sealed with glass ionomer cement (Fuji, GC, Tokyo, Japan). The specimens were stored in an incubator at 95% humidity and 37 °C. After 1 week, the temporary filling material was removed with a round bur (Dentsply Maillefer) under water cooling and followed by the removal of the medicaments using 2 mL of 2.5% NaOCl, 17% EDTA, and sterile saline. Then, the root canals were dried using absorbent paper points. The middle third of each root was cut into two 2.0 ± 0.05 parallel transverse sections (n = 90 slices) in the coronal-to-apical direction using an Isomet low speed saw with continuous water irrigation. Thereafter, the specimens were divided into two subgroups (n = 45 each) according to the calcium silicate cement applied: (i) MTA (ProRoot MTA, Dentsply Tulsa Dental, Tulsa, OK, USA) or (ii) Totalfill Root Repair Material - fast set putty. MTA was mixed according to manufacturer’s instructions. Totalfill Root Repair Material - fast set putty (BC fast set putty) is a premixed ready to use cement. The cements were placed inside the lumen of the slices and condensed using an endodontic plugger on a flat glass plate. Then, a scalpel was used to remove the excess material. Finally, the specimens were wrapped in pieces of gauze soaked in distilled water and stored in an incubator for 1 week.
Push-out bond strength test
The push-out test was conducted using a universal testing machine (Instron testing machine; Model 5965, ITW, MA, USA). The samples were placed on a metal slap containing a central hole to allow free motion of the plunger. Loading on the calcium silicate cement material was applied using a 1.2 mm-diameter plunger at a constant vertical pressure and a speed of 1 mm/min until total bond failure occurred. The plunger tip was positioned to contact the test material only. The highest force applied to materials at the time of dislodgement was recorded in megapascal (MPa) by using the following formula:
The operator who made the measurements was blinded to which sample was matched to which material.
Evaluation of failure patterns
The failure mode in each slice was identified under a digital microscope at 50X magnification (KH-7700, Hirox Co, Tokyo, Japan); these included adhesive failure that occurred at the dentin-material interface, cohesive failure that occurred within the material, or mixed failure, which is the combination of the two failure modes. The operator who examined the slices was blinded to which sample was matched to which material.
Statistical analysis was performed using the Statistical Package for the Social Sciences statistical software (version 21.0, SPSS IBM, Armonk, NY). The data were normally distributed according to Kolmogorov-Smirnov. Then, data were statistically analyzed using two-way analysis of variance (ANOVA). When applicable, Tukey’s post hoc was performed for multiple comparisons. The chi-squared test was used to determine if there was a significant association between the type of failure and the filling material or intracanal medicament. The statistical significance was set at 0.05.
The mean and standard deviations of the push-out bond strength values are presented in Table 1. Two-way ANOVA indicated no significant difference between MTA and BC fast set putty regardless the use of intracanal medicament (p = .20). For both cements, the highest push-out bond strength results were obtained without prior medication (control). However, the application of intracanal medicament did not significantly affect the bond strength of BC fast set putty (p = .63). For MTA, prior application of Ca(OH)2 or mTAP significantly decreased the dislocation resistance (p = .00 and p = .00, respectively) with similar debonding values for both medicaments (p = .61).
Without prior medication (control) or with prior application of Ca(OH)2, there was no significant difference in bond strength between MTA and BC fast set putty (p = .31 and p = .35, respectively). With prior application of mTAP, the BC fast set putty showed significantly higher debonding forces than MTA (p = .01).
Bond failure patterns
Representative microscopic images of the modes of failure are shown in Fig. 1. Few specimens revealed an adhesive type of failure (Table 2). Most specimens in the MTA subgroups showed a nearly equal number of specimens in the cohesive and mixed types while the majority of specimens in all BC fast set putty subgroups revealed the cohesive type of failure. There was no significant association between the type of cement or the intracanal medicament and type of bond failure (p = .07 and p = .81, respectively).
Intracanal medicaments can be used in various endodontic conditions including multiple visit endodontics after trauma or in regenerative endodontics . These medicaments can be applied for different periods varying from 1 week to several months . The American Association of Endodontists recommends the application of intracanal medicament for a minimum of 1 week for clinical regenerative procedures . Furthermore, it has been reported that a 7-day interappointment dressing with Ca(OH)2 was sufficient to reduce intraradicular bacteria to a level that provided a negative culture . However, the long-term exposure to interappointment medicament has been suggested to affect the mechanical properties of root dentin by either collagen degradation in the case of Ca(OH)2 or excessive demineralization in the case of antibiotic pastes that might affect the root fracture resistance . Interim medicaments should be removed before the placement of filling/repair material. Previous studies reported that there is no method (so far) is able to completely remove intracanal medicaments from the root canal system [33, 34]. Therefore, this study aimed to evaluate the effect of Ca(OH)2 and mTAP after short-term application on BC fast set putty in comparison to MTA. Calcium silicate cements might be exposed to dislodging forces when used for perforation repair, apexification or regenerative procedures. One of the requirements of these cements is the ability to persist in place under dislodging forces . The push-out test has been used in previous studies to assess the bond strength of calcium silicate cements to dentin [24, 29]. This method, which was used in this study, has several advantages including practicality and reliability .
The results obtained from this study reveal that regardless of the use of intracanal medicament, there was no significant difference in the bond strength between MTA and BC fast set putty. This result is in line with an earlier study, which reported that there is no significant difference in the dislocation of both materials . Furthermore, the prior application of Ca(OH)2 and mTAP were associated with a significant decrease in the debonding force of MTA. This significant decrease might be explained by the degradation effect of Ca(OH)2 on the dentin collagen matrix caused by the low molecular weight, alkaline pH  and the demineralization effect of antibiotic pastes on dentin because of their acidic nature . The effect of mTAP on the dentin mineral contents was investigated in a recent study and it has been demonstrated that it causes a significant increase in calcium and the calcium/phosphorus ratio . Topcuoglu et al.  found that the prior application of Ca(OH)2 and mTAP do not affect the MTA dislodgement resistance. This discrepancy may be related to the differences in experimental procedures. Topcuoglu et al.  used NaOCl and distilled water to remove the intracanal medicament while NaOCl followed by EDTA and saline were used in the present study. The use of a chelating agent after application of intracanal medicament may cause alteration in the chemical structure of the dentin surface and surface properties that may affect material adhesion to dentin .
Compared with the control, the tested intracanal medicaments did not affect the BC fast set putty bond strength. In the literature, there is no data about the effect of various canal dressings on the dislocation resistance of BC fast set putty to root dentin. Therefore, the results of this study were compared with a study in which the effects of various medicaments on the dislocation resistance of BC sealer was evaluated. Gokturk et al.  found that the application of Ca(OH)2 or an antibiotic paste, which is composed of ciprofloxacin and metronidazole, did not affect the dislodgement resistance of the BC sealer.
The present study demonstrated that the highest push-out bond strength values in both groups were obtained without prior medication. This might be explained by the medicament residue in the canal walls that could act as a barrier and interfere with the material adaptation with dentinal tubules. MTA and BC fast set putty belong to a calcium silicate-based group of materials. During the hydration reaction, endodontic bioceramics release Ca(OH)2, which interacts with phosphates in the tissue fluids to form hydroxyapatite and tag-like structures in dentin . BC fast set putty is premixed by the manufacturer and has a more homogenous and smaller particle size than MTA. Smaller particles might result in easier and deeper penetration into dentinal tubules even in the presence of medicament residue in the canal walls. This may explain the nonsignificant decrease in the debonding force of BC fast set putty compared to the significant decrease in the MTA group.
All specimens were evaluated after the push-out test to determine the mode of failure. Most failures observed in the MTA subgroups were either cohesive or mixed types. This finding is in line with previous studies [23, 29]. The bond failure observed in all BC fast set putty subgroups was predominantly within the material itself. This mode of failure may be explained by the smaller and uniform size of particles in the BC fast set putty, which enabled better penetration into the dentinal tubules and resulted in better adhesion. This finding contrasts with the results of Kadic et al.  who reported that BC-dentin bond failures were usually the mixed type. The conflicting results could be due to the different factors included in the study designs; slices were prepared from the apical third of the root in the Kadic et al.  study instead of the middle root slices used in the present study. Variations in both the number and size of dentinal tubules in the different root canal portions have been reported in the literature . Furthermore, their sections were prepared after material placement inside the root canal that might affect the material adaptation.
Within the limitations of this study, the results might provide information that can aid the clinician in the selection of the best material used in clinical practice. Based on our findings, the dislocation resistance of BC fast set putty is not affected by the prior application of Ca(OH)2 or mTAP. Considering its additional advantages, including ease in handling and that it does not induce color changes in tooth structure, BC fast set putty might be a suitable replacement for MTA after the use of such intracanal medicament to disinfect the root canal, for example, in regenerative endodontics or apexification procedures. However, further experiments are needed to evaluate the effect of intracanal medicaments on other physiochemical properties of BC fast set putty.
Within the conditions of the present study, it can be concluded that Ca(OH)2 and mTAP promoted lower bond strength of MTA to root dentin compared to the control group. However, the BC fast set putty bond strength to dentin was not affected by prior medication with Ca(OH)2 or mTAP.
Availability of data and materials
The datasets for the current study are available from the corresponding author on reasonable request.
Analysis of variance
- BC fast set putty:
TotalFill BC fast set putty
- Ca(OH)2 :
Mineral trioxide aggregate
Modified triple antibiotic paste
Triple antibiotic paste
Kakehashi S, Stanley HR, Fitzgerald RJ. The effects of surgical exposures of dental pulps in germ-free and conventional laboratory rats. Oral Surg Oral Med Oral Pathol. 1965;20:340–9.
Moller AJ, Fabricius L, Dahlen G, Ohman AE, Heyden G. Influence on periapical tissues of indigenous oral bacteria and necrotic pulp tissue in monkeys. Scand J Dent Res. 1981;89:475–84.
Sjogren U, Figdor D, Persson S, Sundqvist G. Influence of infection at the time of root filling on the outcome of endodontic treatment of teeth with apical periodontitis. Int Endod J. 1997;30:297–306.
Siqueira JF, Magalhaes KM, Rocas IN. Bacterial reduction in infected root canals treated with 2.5% NaOCl as an irrigant and calcium hydroxide/camphorated paramonochlorophenol paste as an intracanal dressing. J Endod. 2007;33:667–72.
Hasselgren G, Olsson B, Cvek M. Effects of calcium hydroxide and sodium hypochlorite on the dissolution of necrotic porcine muscle tissue. J Endod. 1988;14:125–7.
Siqueira JF, Lopes HP. Mechanisms of antimicrobial activity of calcium hydroxide: a critical review. Int Endod J. 1999;32:361–9.
Hoshino E, Kurihara-Ando N, Sato I, Uematsu H, Sato M, Kota K, Iwaku M. In-vitro antibacterial susceptibility of bacteria taken from infected root dentine to a mixture of ciprofloxacin, metronidazole and minocycline. Int Endod J. 1996;29:125–30.
Bose R, Nummikoski P, Hargreaves K. A retrospective evaluation of radiographic outcomes in immature teeth with necrotic root canal systems treated with regenerative endodontic procedures. J Endod. 2009;35:1343–9.
Ozan U, Er K. Endodontic treatment of a large cyst-like periradicular lesion using a combination of antibiotic drugs: a case report. J Endod. 2005;31:898–900.
Kim JH, Kim Y, Shin SJ, Park JW, Jung IY. Tooth discoloration of immature permanent incisor associated with triple antibiotic therapy: a case report. J Endod. 2010;36:1086–91.
Thibodeau B, Trope M. Pulp revascularization of a necrotic infected immature permanent tooth: case report and review of the literature. Pediatr Dent. 2007;29:47–50.
Dawood AE, Parashos P, Wong RHK, Reynolds EC, Manton DJ. Calcium silicate-based cements: composition, properties, and clinical applications. J Investig Clin Dent. 2017;8(2):e12195.
Lee SJ, Monsef M, Torabinejad M. Sealing ability of a mineral trioxide aggregate for repair of lateral root perforations. J Endod. 1993;19:541–4.
Sousa CJ, Loyola AM, Versiani MA, Biffi JC, Oliveira RP, Pascon EA. A comparative histological evaluation of the biocompatibility of materials used in apical surgery. Int Endod J. 2004;37:738–48.
Nair PN, Duncan HF, Ford TRP, Luder HU. Histological, ultrastructural and quantitative investigations on the response of healthy human pulps to experimental capping with mineral trioxide aggregate: a randomized controlled trial. 2008. Int Endod J. 2009;42:422–44.
Guo YJ, Du TF, Li HB, Shen Y, Mobuchon C, Hieawy A, Wang ZJ, Yang Y, Ma J, Haapasalo M. Physical properties and hydration behavior of a fast-setting bioceramic endodontic material. BMC Oral Health. 2016;16:23.
Ber BS, Hatton JF, Stewart GP. Chemical modification of proroot mta to improve handling characteristics and decrease setting time. J Endod. 2007;33:1231–4.
Alsubait S, Al-Haidar S, Al-Sharyan N. A comparison of the discoloration potential for endosequence bioceramic root repair material fast set putty and proroot mta in human teeth: an in vitro study. J Esthet Restor Dent. 2017;29:59–67.
Zhang W, Peng B. Tissue reactions after subcutaneous and intraosseous implantation of iRoot SP, MTA and AH plus. Dent Mater J. 2015;34:774–80.
Shokouhinejad N, Nekoofar MH, Razmi H, Sajadi S, Davies TE, Saghiri MA, Gorjestani H, Dummer PM. Bioactivity of endosequence root repair material and bioaggregate. Int Endod J. 2012;45:1127–34.
Walsh RM, Woodmansey KF, Glickman GN, He J. Evaluation of compressive strength of hydraulic silicate-based root-end filling materials. J Endod. 2014;40:969–72.
Shahi S, Rahimi S, Yavari HR, Samiei M, Janani M, Bahari M, Abdolrahimi M, Pakdel F, Aghbali A. Effects of various mixing techniques on push-out bond strengths of white mineral trioxide aggregate. J Endod. 2012;38:501–4.
Topcuoglu HS, Arslan H, Akcay M, Saygili G, Cakici F, Topcuoglu G. The effect of medicaments used in endodontic regeneration technique on the dislocation resistance of mineral trioxide aggregate to root canal dentin. J Endod. 2014;40:2041–4.
Nagas E, Cehreli ZC, Uyanik MO, Vallittu PK, Lassila LV. Effect of several intracanal medicaments on the push-out bond strength of ProRoot MTA and biodentine. Int Endod J. 2016;49:184–8.
Turk T, Ozisik B, Aydin B. Time-dependent effectiveness of the intracanal medicaments used for pulp revascularization on the dislocation resistance of MTA. BMC Oral Health. 2015;15:130.
Schneider SW. A comparison of canal preparations in straight and curved root canals. Oral Surg Oral Med Oral Pathol. 1971;32:271–5.
Fundaoglu Küçükekenci F, Çakici F, Küçükekenci AS. Spectrophotometric analysis of discoloration and internal bleaching after use of different antibiotic pastes. Clin Oral Investig. 2019;23:161–7.
Athanassiadis B, Abbott PV, Walsh LJ. The use of calcium hydroxide, antibiotics and biocides as antimicrobial medicaments in endodontics. Aust Dent J. 2007;52:S64–82.
Alsubait SA, Hashem Q, AlHargan N, AlMohimeed K, Alkahtani A. Comparative evaluation of push-out bond strength of ProRoot MTA, bioaggregate and biodentine. J Contemp Dent Pract. 2014;15:336–40.
American Association of Endodontists (2018) clinical considerations for a regenerative procedure. https://f3f142zs0k2w1kg84k5p9i1o-wpengine.netdna-ssl.com/specialty/wp-content/uploads/sites/2/2018/06/ConsiderationsForRegEndo_AsOfApril2018.pdf. Accessed 4 January 2018.
Sjogren U, Figdor D, Spangberg L, Sundqvist G. The antimicrobial effect of calcium hydroxide as a short-term intracanal dressing. Int Endod J. 1991;24:119–25.
Yassen GH, Chu TM, Eckert G, Platt JA. Effect of medicaments used in endodontic regeneration technique on the chemical structure of human immature radicular dentin: an in vitro study. J Endod. 2013;39:269–73.
Wigler R, Dvir R, Weisman A, Matalon S, Kfir A. Efficacy of XP-endo finisher files in the removal of calcium hydroxide paste from artificial standardized grooves in the apical third of oval root canals. Int Endod J. 2017;50:700–5.
Chou K, George R, Walsh LJ. Effectiveness of different intracanal irrigation techniques in removing intracanal paste medicaments. Aust Endod J. 2014;40:21–5.
Shokouhinejad N, Yazdi KA, Nekoofar MH, Matmir S, Khoshkhounejad M. Effect of acidic environment on dislocation resistance of endosequence root repair material and mineral trioxide aggregate. J Dent (Tehran). 2014;11:161–6.
Yassen GH, Vail MM, Chu TG, Platt JA. The effect of medicaments used in endodontic regeneration on root fracture and microhardness of radicular dentine. Int Endod J. 2013;46:688–95.
Simsek H, Coruh M, Cakici F, Kucukekenci FF, Gurbuz T, Cakici EB. Investigation of mineral content of root canal dentin after the application of various antibiotic paste using energy-dispersive X-ray detector. Microsc Res Tech. 2019;82:144–8.
Yassen GH, Sabrah AH, Eckert GJ, Platt JA. Effect of different endodontic regeneration protocols on wettability, roughness, and chemical composition of surface dentin. J Endod. 2015;41:956–60.
Gokturk H, Bayram E, Bayram HM, Aslan T, Ustun Y. Effect of double antibiotic and calcium hydroxide pastes on dislodgement resistance of an epoxy resin-based and two calcium silicate-based root canal sealers. Clin Oral Investig. 2017;21:1277–82.
Trope M, Bunes A, Debelian G. Root filling materials and techniques: bioceramics a new hope? Endod Top. 2015;32:86–96.
Kadic S, Baraba A, Miletic I, Ionescu A, Brambilla E, Malcic AI, Gabric D. Push-out bond strength of three different calcium silicate-based root-end filling materials after ultrasonic retrograde cavity preparation. Clin Oral Investig. 2018;22:1559–65.
Lo Giudice G, Cutroneo G, Centofanti A, et al. Dentin morphology of root canal surface: a quantitative evaluation based on a scanning electronic microscopy study. Biomed Res Int. 2015. https://doi.org/10.1155/2015/164065.
This study was supported by the College of Dentistry Research Center (CDRC) at King Saud University (FR0446) in Riyadh, Saudi Arabia. The fund is directed toward the lab work and equipment used for this study.
Ethics approval and consent to participate
This work was approved by the Institutional Review Board (IRB) of King Saud University in Riyadh, Saudi Arabia (E-18-3078). The consent of the participants for the use of their extracted teeth was waived by IRB committee because the teeth were collected anonymously as part of a regular dental treatment and were not used for genetic testing or any other tests except for the in vitro evaluation of the push-out bond strength of dental materials.
Consent for publication
The authors declare that they have no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Alsubait, S., Alsaad, N., Alahmari, S. et al. The effect of intracanal medicaments used in Endodontics on the dislocation resistance of two calcium silicate-based filling materials. BMC Oral Health 20, 57 (2020). https://doi.org/10.1186/s12903-020-1044-6
- Bond strength
- Calcium hydroxide
- Calcium silicate-based cements
- Material testing
- Modified triple antibiotic paste