Skip to main content

Core of the saliva microbiome: an analysis of the MG-RAST data



Oral microbiota is considered as the second most complex in the human body and its dysbiosis can be responsible for oral diseases. Interactions between the microorganism communities and the host allow establishing the microbiological proles. Identifying the core microbiome is essential to predicting diseases and changes in environmental behavior from microorganisms.


Projects containing the term “SALIVA”, deposited between 2014 and 2019 were recovered on the MG-RAST portal. Quality (Failed), taxonomic prediction (Unknown and Predicted), species richness (Rarefaction), and species diversity (Alpha) were analyzed according to sequencing approaches (Amplicon sequencing and Shotgun metagenomics). All data were checked for normality and homoscedasticity. Metagenomic projects were compared using the Mann–Whitney U test and Spearman's correlation. Microbiome cores were inferred by Principal Component Analysis. For all statistical tests, p < 0.05 was used.


The study was performed with 3 projects, involving 245 Amplicon and 164 Shotgun metagenome datasets. All comparisons of variables, according to the type of sequencing, showed significant differences, except for the Predicted. In Shotgun metagenomics datasets the highest correlation was between Rarefaction and Failed (r =  − 0.78) and the lowest between Alpha and Unknown (r =  − 0.12). In Amplicon sequencing datasets, the variables Rarefaction and Unknown (r = 0.63) had the highest correlation and the lowest was between Alpha and Predicted (r =  − 0.03). Shotgun metagenomics datasets showed a greater number of genera than Amplicon. Propionibacterium, Lactobacillus, and Prevotella were the most representative genera in Amplicon sequencing. In Shotgun metagenomics, the most representative genera were Escherichia, Chitinophaga, and Acinetobacter.


Core of the salivary microbiome and genera diversity are dependent on the sequencing approaches. Available data suggest that Shotgun metagenomics and Amplicon sequencing have similar sensitivities to detect the taxonomic level investigated, although Shotgun metagenomics allows a deeper analysis of the microorganism diversity. Microbiome studies must consider characteristics and limitations of the sequencing approaches. Were identified 20 genera in the core of saliva microbiome, regardless of the health condition of the host. Some bacteria of the core need further study to better understand their role in the oral cavity.

Peer Review reports


Metagenomic is a technique for accessing non-cultivable microorganisms DNA from environmental samples [1]. Since the development of New Generation Sequencing (NGS), this technique has been widely used in a number of scientific studies [2,3,4]. However, one of the challenges in metagenomics is to work with a large volume of data generated by sequencing and analysis. In bioinformatics, sequencing data must be deposited in a public database for wide access to be published in a scientific article. Therefore, since 2008 several specialized databases have allowed the deposit of raw and analyzed data from metagenomics projects [5, 6]. One of the pioneers in storage platforms for metagenomic data analysis is the public access portal MG-RAST [6].

The MG-RAST portal has deposited projects generated from different metagenomics approaches: Amplicon sequencing, Shotgun metagenomics and Metatranscriptomic. Amplicon sequencing (or metabarcoding) is done using the products of the polymerase chain reaction (PCR) that amplify the marker genes, such as 16S rRNA, 23S rRNA and 18S rRNA. Shotgun metagenomics has been used for total DNA sequencing from environmental samples while Metatranscriptomic has been used for sequencing all RNA extracted from investigated samples.

The oral microbiota refers to the collection of microorganisms that inhabit the oral cavity in different locations, such as the tongue, saliva and teeth [7]. Saliva has been shown to be a biological material capable of reflecting the dynamics of health conditions and metabolic, immunological or infectious diseases, reflecting dysbiosis of local and systemic origin, as observed in dental caries, periodontal diseases, diabetes, rheumatoid arthritis [8], cancer and, more recently, SARS-COV-2 [9, 10]. Obtaining salivary samples is simple, easy to perform, non-invasive, does not cause discomfort to the patient, is inexpensive, and representative of the oral environment. Saliva, like other biological fluids, contains DNA, RNA, proteins, and metabolic products, which are components of the host and its microbiota and their interactions. Despite the ease of its collection, knowledge of the behavior of the salivary microbiota and other constituents is still a challenge. Studies using sophisticated analysis such as the NGS, allow the investigation of differences in the bacterial profile in patients with oral diseases, such as caries and periodontitis, compared to healthy individuals [11].

The oral human microbiome, considered as the second most complex, is composed of more than 700 species [12] of which 54% are cultivable, 14% cultivable but unidentifiable, in addition to 32% of microorganisms unable to be cultured and identified [13].

Recent studies have proven the existence of an intrinsic relationship between the environment conditions and microbiome profiles [3, 13, 14]. Oral microbiota dysbiosis can be responsible for oral diseases, such as caries, plaque and periodontics [15,16,17]. Host-microbiological interactions allow establishing differences in microbiome profiles due to physiological and pathological conditions [18]. During the last decades, the development of methodologies and analyses for the identification and characterization of microbiomes has made it possible to predict diseases associated with changes in the environment and their reflexes in the microbiota, particularly those that share the same niche [19].

Description of microorganisms that share the same niche is called a core [19]. Identifying the core microbiome is essential to define how “healthy” this environment is [19]. The literature points out that the 10% prevalent microorganisms in the core must be considered dominant. On the other hand, the 65% less predominant should be considered rare [20].

Therefore, the relationship of this core with the environment, predicting diseases, and changes in environmental behavior from microorganisms not belonging to the core need further investigation [19].

Main text


This study aimed to investigate the core of the oral microbiome in saliva samples, regardless of host conditions by using the MG-RAST portal database.

Identifiers of metagenomes deposited between 2014 and 2019, containing as keyword the term "saliva" in the Material variable (material = ’SALIVA’) were selected for the study. A python in-house script was developed to extract, transform and load metadata from selected metagenomes to be filtered and analyzed. In order to assess the differences between sequencing approaches, only metagenomic projects that contained Amplicon sequencing and Shotgun metagenomics approaches were used in this study. In addition, only projects that had more than 3 metagenomes in each of the approaches were used due to statistical inferences.

Metagenomes were analyzed according to quality (Failed) and taxonomic prediction (Unknown and Predicted). In addition, metagenomes were analyzed according to species richness (Rarefaction) and species diversity (Alpha) (Table 1). Taxonomic data were recovered from the projects selected for the study of the core of the microbiome. Genus level was chosen for the analysis because Amplicon sequencing approach is more consistent for this taxonomic level [21].

Table 1 Variables recovered in MG-RAST

All data were checked for normality and homoscedasticity by Shapiro–Wilk test. Amplicon sequencing and Shotgun metagenomics data were compared using the Mann–Whitney U test. Associations among Failed, Unknown, Predicted, Alpha, and Rarefaction were performed by Spearman’s correlation. Microbiome cores were obtained and analyzed by Principal Component Analysis (PCA), to evaluate the most representative organisms of Amplicon sequencing and Shotgun metagenomics.

To allow a comparison between the results obtained in this study and the current literature, representative data of the oral microbiome from the expanded Human Oral Microbiome Database (eHOMD) [22] were used. eHOMD data were retrieved using the Taxon Table available at After setting the Body Site filter field by checking only the Oral option, the text file was downloaded. Only the information in the Genus column was considered. Then the redundancies were removed and the remaining genera were considered the microbiome core of eHOMD. Microbiome cores obtained in the Amplicon sequencing and Shotgun metagenomics were compared with the microbiome core of the eHOMD by Venn diagram.

Statistical analysis was performed using the software R v3.6.1 [23]. The libraries dplyr, ggplot2, reshape2, and data.table were used in the descriptive analysis and correlation study. The factoextra library was used in the PCA. The value of p < 0.05 was used in all tests.

Python and R scripts are available on GitHub (

In order to obtain more information about the selected projects, a search was carried out on PUBMED using the information of the principal investigator and the description of the project, both available on MG-RAST.


The survey in MG-RAST identified 621 metagenomes. Python script recovered 476 metagenomes, distributed in 12 sequencing projects, of which 332 metagenomes were Amplicon sequencing, 142 Shotgun metagenomics, and 2 Metatranscriptomes. The remaining 145 metagenomes had no metadata available and were discarded, as well as the recovered metatranscriptomes.

Projects that contained metagenomes from both sequencing approaches were selected. In addition, 1 project that had only 1 metagenome for each approach was discarded. In this way, 3 projects with 245 Amplicon sequencing metagenomes and 164 Shotgun metagenomics metagenomes were used in this study (Table 2).

Table 2 Number of metagenomes by project and sequencing approaches

Figure 1 shows the results of the descriptive analysis of the numerical variables. For the Amplicon sequencing datasets there was no sequence with quality problems. All variables of both approaches had a non-Gaussian distribution, with the exception of the Alpha in Shotgun metagenomics. All comparisons of variables, according to the sequencing approaches, showed significant differences (p < 0.05), except for the Predicted (p = 0.4307).

Fig. 1
figure 1

Descriptive analysis. Boxplot shows the data distribution of Failed, Unknown, Predicted, Alpha and Rarefaction variables, comparing (a) Amplicon sequencing and (b) Shotgun metagenomics approaches. Normality analysis was performed using the Shapiro Wilk test and the Mann–Whitney U test evaluated the differences of the variables between the sequencing approaches. All variables showed a significant difference (p < 0.05) between Amplicon sequencing and Shotgun metagenomics, except Predicted. Note that Failed did not present any data in the Amplicon sequencing

Correlation study of the five variables showed divergences between sequencing approaches (Fig. 2). Most of the correlations of the variables in Amplicon sequencing datasets were positive, in contrast to what was observed in Shotgun metagenomics. In Shotgun datasets, the highest correlation was between Rarefaction and Failed (r =  − 0.78) and the lowest between Alpha and Unknown (r =  − 0.12). In Amplicon datasets, Rarefaction and Unknown (r = 0.63) had the highest correlation and the lowest was between Alpha and Predicted (r =  − 0.03).

Fig. 2
figure 2

Correlation analysis. The Spearman’s test evaluated the associations between Failed, Unknown, Predicted, Alpha, and Rarefaction variables of a Amplicon sequencing and b Shotgun metagenomics. All associations were positive in Amplicon sequencing. In the Shotgun metagenomics, Failed and Unknown were negatively associated with Predicted, Alpha, and Rarefaction

In the PCA, microbiome cores with different genera were found between Amplicon sequencing and Shotgun metagenomics. Number of genera obtained by the Shotgun dataset was greater than that observed in the Amplicon dataset. The top 10 genera showed that only Prevotella and Streptococcus are representative in cores of both approaches. Propionibacterium, Lactobacillus and Prevotella were the most representative genera in Amplicon sequencing. On the other hand, Escherichia, Chitinophaga, and Acinetobacter (Figs. 3 and 4) were the most representative genera in Shotgun metagenomics. The genera present in the microbiome core of both Shotgun and Amplicon are listed in Additional file 1: S1.

Fig. 3
figure 3

Principal Components Analysis. Bi-plot showing the representativeness of the genera present in the core of both approaches: a Amplicon sequencing and b Shotgun metagenomics. Graphs show the dimensions with greatest variance.

Fig. 4
figure 4

Top 10 bacteria in the human saliva microbiome. Contribution of the main genera in dimensions 1 and 2 of the Principal Component Analysis (PCA) of the Amplicon sequencing and Shotgun metagenomics approaches. Amplicon sequencing: a dimension 1 and b dimension 2. Shotgun metagenomics: c dimension 1 and d dimension 2

By comparing Shotgun metagenomics, Amplicon sequencing and eHOMD datasets a common or shared microbiome core containing 20 genera was found (Table 3). eHOMD and Shotgun metagenomics datasets share a microbiome core composed of 39 genera, and eHOMD and Amplicon sequencing share a core with only 9 genera. Shotgun and Amplicon datasets share a core containing 7 genera (Fig. 5). The complete genera names sharing the different microbiome cores is in Additional file 2: S2.

Table 3 Bacteria genera of human saliva microbiome core and their relationship with oral diseases
Fig. 5
figure 5

Core of saliva microbiomes. Venn diagram showing microbiome cores shared among Amplicon sequencing, Shotgun metagenomics, and eHOMD

Search in PUBMED identified 12 articles published between 2011 and 2015, with the PMIDs: 25994215, 25861745, 25020228, 24981669, 24903519, 24846382, 24646696, 23598790, 22583485, 2254158393, 21149389, and 205478343.


Bacteria are highly prevalent microorganisms in the microbiota and play an important role in oral homeostasis [41]. The abundance of some bacteria may indicate dysbiosis of the oral microbiome [25, 35]. Identification of the core is inferred from the study of different microbiomes and allows to reveal the conditions of the hosts according to the presence/absence or predominance of some species over others. However, there is a set of bacteria that, regardless of the condition of the host, can be part of the core of all these microbiomes. Similar to the concept of housekeeping genes, which conceptualize the genes essential to the life of an organism, a set of bacteria from the oral microbiome, regardless of the health status of the host, can be inferred as that essential to the symbiosis between the microorganisms of the oral cavity.

In this study, 20 genera of bacteria were found in more than 450 metagenomes (Fig. 5) deposited in public databases and which, regardless of the health condition of the host, are present in the core of the oral microbiome.

In both Amplicon sequencing and Shotgun metagenomics cores, genera of bacteria already associated with caries and periodontal diseases were found, such as Streptococcus, Lactobacillus and Prevotella [10, 42].

The saliva of individuals with high caries experience is associated with a high salivary abundance of Streptococcus and countless species of Lactobacillus in addition to other bacteria capable of degrading sugars and forming extracellular polysaccharides [18]. Samples from healthy individuals with low caries experience were associated with a greater abundance of the genera Neisseria, Haemophilus, and Fusobacterium, of which most species of this genera only ferment sugar slightly [43]. According to Tanner et al. [18], the composition of saliva in the oral cavity is one of the main risk factors associated with caries. Biofilm dysbiosis results in an increase in acidogenic and aciduric species, capable of modulating the core components in the biofilm. While in cases of gingivitis, the increase in the amount of plaque around the gingival margin induces the inflammatory response in the host, leading to increased levels of anaerobic bacteria, including Gram-negative proteolytic species, especially those belonging to the Prevotella, Porphyromonas, Tannerella, Fusobacterium and Treponema genera [44].

Identification of Corynebacterium, Escherichia, Pseudomonas and Shigella suggests that genera with pathogenic potential may also be part of the core of the oral microbiome obtained by salivary samples. Chitinophaga was a recently described taxon and was observed only in Shotgun metagenomics. This genus was highly representative, as well as Escherichia, Acinetobacter, Streptococcus, and Shigella (Fig. 3). The pathogenic potential of the genus Chitinophaga has already been reported [45, 46]. However, its role in the oral microbiome is still unknown.

Amplicon sequencing metadata analysis showed inconsistent behavior (Fig. 2). Only Rarefaction and Unknown had correlation greater than 0.50 (r = 0.63). The expected behavior was of inverse correlation. The greatest number of non-inferred sequences determines the smallest number of potentially discovered organisms.

On the other hand, Shotgun metagenomics presented results as expected, which can be exemplified by inverse correlation between Failure and Rarefaction. The largest number of sequences with quality failure determines the smallest number of sequences to be inferred, which affects the rarefaction curve. This was exactly the behavior observed in Shotgun metagenomics for these variables (r =  − 0.78).

These behaviors in different approaches certainly influenced the comparative study between them (Fig. 1). Furthermore, it was observed in the Amplicon sequencing dataset that all projects did not present sequences with quality failures (Failed = 0). This result was unexpected. Even Amplicon sequencing can have quality failures in the sequencing process. However, the Predicted was the only one that did not show difference between the approaches, showing results similar to those observed by [47], who investigated the microbial composition of the human intestine.

Results obtained by Shotgun metagenomics allowed a more complex characterization of the microbiome, with the identification of greater diversity and at the taxonomic level of species, when compared to Amplicon sequencing which uses regions of the gene with variability to identify down to the genus level [48].

According to the literature [47, 49], the PCA identified a greater number of representative genera in the Shotgun dataset than in the Amplicon dataset (Fig. 3). The differences between them may explain the findings. In Shotgun metagenomics, the DNA of all the organisms in the sample is extracted and sequenced directly. On the other hand, in Amplicon sequencing, only the DNA fragments that were aligned to the primer will be sequenced. The choice of primer seems to be a crucial factor to avoid bias in taxonomic analysis [50].

The specificity of primers may restrict the set of microorganisms found in studies of Amplicon sequencing. Thus, the choice of the sequencing method as well as the selection of primers are important characteristics to be considered in the analysis of microbiome studies [51].

Microbiome studies comparing the two sequencing methods for the same samples suggest that their results might be comparable. In this study, we observed that the data produced by Shotgun metagenomics of salivary samples available on the MG-RAST platform can provide the identification of a greater number of genera, evidencing the complexity of the oral microbiome, either by the diversity of genera or by the role they may play in the salivary microbiome [50].

These results should be interpreted with caution, since only the presence of the genera does not determine the condition of the host. Other characteristics such as abundance and interaction between genera have a relevant role in the association of the microbiota with the condition of the host [12].

Metagenomics projects deposited in public databases such as eHOMD and MG-RAST do not always provide information on the health conditions of the host, DNA/RNA extraction techniques or other information that might infer microbiome-host relationships.

Studies identified using the information of the principal investigator suggest that they correspond to the data obtained in the MG-RAST. However, it is not possible to specify whether such articles refer to data investigated in this study. According to the MG-RAST pipeline guideline (, it is not possible to carry out analyses of eukaryotes or viruses, which suggests that the DNA/RNA extraction method of the selected projects allows inferring the bacterial microbiota.


This study demonstrated that in the microbiota representative of human saliva, genera of pathogenic bacteria observed in oral diseases were identified, but not limited to them.

Core of the salivary microbiome and genera diversity are dependent on the sequencing approaches. Available data suggest that Shotgun metagenomics and Amplicon sequencing have similar sensitivities to detect the taxonomic level investigated, although Shotgun metagenomics allows a deeper analysis of the microorganism diversity.

The choice of metagenomics approaches must consider their characteristics and limitations. Shotgun metagenomics sequencing can provide a great contribution to the knowledge of the composition of the salivary microbiota, identification of markers for diagnosis and identification of profiles capable of defining health or disease conditions. On the other hand, Amplicon sequencing can be an efficient and low-cost choice in studies in which the microorganism of interest is already known. It can also be used for further verification of results obtained by Shotgun metagenomics.

Availability of data and materials

Data was deposited in the Mendeley Data ( Scripts are available at



New generation sequencing.


Polymerase chain reaction.


Principal components analysis.


Expanded Human Oral Microbiome Database


  1. Handelsman J, Rondon MR, Brady SF, Clardy J, Goodman RM. Molecular biological access to the chemistry of unknown soil microbes: a new frontier for natural products. Chem Biol. 1998;5:R245–9.

    Article  PubMed  Google Scholar 

  2. Tierney BT, et al. The landscape of genetic content in the gut and oral human microbiome. Cell Host Microbe. 2019;26:283-295.e8.

    Article  PubMed  PubMed Central  Google Scholar 

  3. Fróes AM, da Mota FF, Cuadrat RRC, Dávila AMR. Distribution and classification of serine β-lactamases in Brazilian hospital sewage and other environmental metagenomes deposited in public databases. Front Microbiol. 2016;7:1790.

    Article  PubMed  PubMed Central  Google Scholar 

  4. Mardis ER. Next-generation DNA sequencing methods. Annu Rev Genom Hum Genet. 2008;9:387–402.

    Article  Google Scholar 

  5. Leinonen R, Sugawara H, Shumway M. The sequence read archive. Nucl Acids Res. 2011;39:D19-21.

    Article  PubMed  Google Scholar 

  6. Meyer F, et al. The metagenomics RAST server-a public resource for the automatic phylogenetic and functional analysis of metagenomes. BMC Bioinform. 2008;9:386.

    Article  Google Scholar 

  7. Handsley-Davis M, Jamieson L, Kapellas K, Hedges J, Weyrich LS. The role of the oral microbiota in chronic non-communicable disease and its relevance to the Indigenous health gap in Australia. BMC Oral Health. 2020;20:327.

    Article  PubMed  PubMed Central  Google Scholar 

  8. Zhang X, et al. The oral and gut microbiomes are perturbed in rheumatoid arthritis and partly normalized after treatment. Nat Med. 2015;21:895–905.

    Article  PubMed  Google Scholar 

  9. Fini MB. Oral saliva and COVID-19. Oral Oncol. 2020;108:104821.

    Article  Google Scholar 

  10. Streckfus CF. Advances in salivary diagnostics. Advances in salivary diagnostics. Berlin: . Springer; 2015.

    Book  Google Scholar 

  11. Suárez Moya A. Microbioma y secuenciación masiva. Span J Chemother. 2017;30:305–11.

    Google Scholar 

  12. Dewhirst FE, et al. The human oral microbiome. J Bacteriol. 2010;192:5002–17.

    Article  PubMed  PubMed Central  Google Scholar 

  13. Caselli E, et al. Defining the oral microbiome by whole-genome sequencing and resistome analysis: the complexity of the healthy picture. BMC Microbiol. 2020.

    Article  PubMed  PubMed Central  Google Scholar 

  14. Wang S, Yan Z, Wang P, Zheng X, Fan J. Comparative metagenomics reveals the microbial diversity and metabolic potentials in the sediments and surrounding seawaters of Qinhuangdao mariculture area. PLoS ONE. 2020;15:e0234128.

    Article  PubMed  PubMed Central  Google Scholar 

  15. Nibali L, et al. Differences in the periodontal microbiome of successfully treated and persistent aggressive periodontitis. J Clin Periodontol. 2020;47:980–90.

    Article  PubMed  Google Scholar 

  16. Nomura Y, Otsuka R, Hasegawa R, Hanada N. Oral microbiome of children living in an isolated area in Myanmar. Int J Environ Res Public Health. 2020;17:4033–44.

    Article  PubMed Central  Google Scholar 

  17. Papapanou PN, et al. Subgingival microbiome and clinical periodontal status in an elderly cohort: the WHICAP ancillary study of oral health. J Periodontol. 2020;91:S56–67.

    Article  PubMed  PubMed Central  Google Scholar 

  18. Tanner ACR, Kressirer CA, Rothmiller S, Johansson I, Chalmers NI. The caries microbiome: implications for reversing dysbiosis. Rev Adv Dent Res. 2018;29:78–85.

    Article  Google Scholar 

  19. Shade A, Handelsman J. Beyond the venn diagram: the hunt for a core microbiome. Environ Microbiol. 2012;14:4–12.

    Article  PubMed  Google Scholar 

  20. Ugland KI, Gray JS. Lognormal distributions and the concept of community equilibrium. Oikos. 1982;39:171–8.

    Article  Google Scholar 

  21. Ranjan R, Rani A, Metwally A, McGee HS, Perkins DL. Analysis of the microbiome: advantages of whole genome shotgun versus 16S amplicon sequencing. Biochem Biophys Res Commun. 2016;469:967–77.

    Article  PubMed  Google Scholar 

  22. Escapa IF, et al. New insights into human nostril microbiome from the expanded human oral microbiome database (eHOMD): a resource for the microbiome of the human aerodigestive tract. mSystems. 2018;3:e00187-18.

    Article  PubMed  PubMed Central  Google Scholar 

  23. R Core Team. R: a language and environment for statistical computing. (R Foundation for Statistical Computing, 2020).

  24. Zhang W, et al. Salivary microbial dysbiosis is associated with systemic inflammatory markers and predicted oral metabolites in non-small cell lung cancer patients. J Cancer. 2019;10:1651–62.

    Article  PubMed  PubMed Central  Google Scholar 

  25. Dafar A, Bankvall M, Çevik-Aras H, Jontell M, Sjöberg F. Lingual microbiota profiles of patients with geographic tongue. J Oral Microbiol. 2017;9:1355206.

    Article  PubMed  PubMed Central  Google Scholar 

  26. Sharma D, et al. Saliva microbiome in primary Sjögren’s syndrome reveals distinct set of disease-associated microbes. Oral Dis. 2020;26:295–301.

    Article  PubMed  Google Scholar 

  27. Pereira CA, et al. Opportunistic microorganisms in individuals with lesions of denture stomatitis. Diagn Microbiol Infect Dis. 2013;76:419–24.

    Article  PubMed  Google Scholar 

  28. Guerrero-Preston R, et al. 16S rRNA amplicon sequencing identifies microbiota associated with oral cancer, Human Papilloma Virus infection and surgical treatment. Oncotarget. 2016;7:51320–34.

    Article  PubMed  PubMed Central  Google Scholar 

  29. de Melo F, Milanesi FC, Angst PDM, Oppermann RV. A systematic review of the microbiota composition in various peri-implant conditions: data from 16S rRNA gene sequencing. Arch Oral Biol. 2020;117:104776.

    Article  PubMed  Google Scholar 

  30. Yamashita Y, Takeshita T. The oral microbiome and human health. J Oral Sci. 2017;59:201–6.

    Article  PubMed  Google Scholar 

  31. Kakabadze MZ, Paresishvili T, Karalashvili L, Chakhunashvili D, Kakabadze Z. Oral microbiota and oral cancer: review. Oncol Rev. 2020;14:476.

    Article  PubMed  PubMed Central  Google Scholar 

  32. Sun X, et al. Alteration of salivary microbiome in periodontitis with or without type-2 diabetes mellitus and metformin treatment. Sci Rep. 2020;10:1–14.

    Article  Google Scholar 

  33. Yu FY, et al. Dysbiosis of saliva microbiome in patients with oral lichen planus. BMC Microbiol. 2020;20:75.

    Article  PubMed  PubMed Central  Google Scholar 

  34. Gao X, Jiang S, Koh D, Hsu CYS. Salivary biomarkers for dental caries. Periodontol. 2016;2000(70):128–41.

    Article  Google Scholar 

  35. Ratna Sudha M, Neelamraju J, Surendra Reddy M, Kumar M. Evaluation of the effect of probiotic bacillus coagulans unique IS2 on mutans streptococci and lactobacilli levels in saliva and plaque: a double-blind, randomized, placebo-controlled study in children. Int J Dent. 2020;2020:8891708.

    Article  PubMed  PubMed Central  Google Scholar 

  36. Esberg A, et al. Corynebacterium matruchotii demography and adhesion determinants in the oral cavity of healthy individuals. Microorganisms. 2020;8:1780.

    Article  PubMed Central  Google Scholar 

  37. Gennari CGM, Sperandeo P, Polissi A, Minghetti P, Cilurzo F. Lysozyme mucoadhesive tablets obtained by freeze-drying. J Pharm Sci. 2019;108:3667–74.

    Article  PubMed  Google Scholar 

  38. Cortela DCB, Souza Junior ALD, Virmond MCL, Ignotti E. Inflammatory mediators of leprosy reactional episodes and dental infections: a systematic review. Mediators Inflamm. 2015;1–15.

  39. Vieira AR, et al. Profiling microorganisms in whole saliva of children with and without dental caries. Clin Exp Dent Res. 2019;5:438–46.

    Article  PubMed  PubMed Central  Google Scholar 

  40. Ogle OE. Salivary gland diseases. Dent Clin North Am. 2020;64:87–104.

    Article  PubMed  Google Scholar 

  41. Zhang Y, et al. Human oral microbiota and its modulation for oral health. Biomed Pharmacother. 2018;99:883–93.

    Article  PubMed  Google Scholar 

  42. Belstrøm D. The salivary microbiota in health and disease. J Oral Microbiol. 2020;12:1–7.

    Article  Google Scholar 

  43. Belstrøm D, et al. Salivary microbiota in individuals with different levels of caries experience. J Oral Microbiol. 2017;9:1–8.

    Article  Google Scholar 

  44. Willmann C, et al. Oral health status in historic population: Macroscopic and metagenomic evidence. PLoS ONE. 2018;13:1–18.

    Article  Google Scholar 

  45. Tran TLQ, et al. Chitinophaga vietnamensis sp. Nov., a multi-drug resistant bacterium infecting humans. Int J Syst Evol Microbiol. 2020;70:1758–68.

    Article  PubMed  Google Scholar 

  46. Crémet L, et al. Chitinophaga terrae bacteremia in human. Emerg Infect Dis. 2009;15:1134–5.

    Article  PubMed  PubMed Central  Google Scholar 

  47. Laudadio I, et al. Quantitative assessment of shotgun metagenomics and 16S rDNA Amplicon sequencing in the study of human gut microbiome. OMICS J Integr Biol. 2018;22:248–54.

    Article  Google Scholar 

  48. Wade WG. The oral microbiome in health and disease. Pharmacol Res. 2013;69:137–43.

    Article  PubMed  Google Scholar 

  49. Jovel J, et al. Characterization of the gut microbiome using 16S or shotgun metagenomics. Front Microbiol Wwwfrontiersinorg. 2016;1:459–76.

    Google Scholar 

  50. Rintala A, et al. Gut microbiota analysis results are highly dependent on the 16s rRNA gene target region, whereas the impact of DNA extraction is minor. J Biomol Tech. 2017;28:19–30.

    Article  PubMed  PubMed Central  Google Scholar 

  51. Clarridge JE. Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases. Clin Microbiol Rev. 2004;17:840–62.

    Article  PubMed  PubMed Central  Google Scholar 

Download references


Rio de Janeiro State University, Piracicaba Dental School, State University of Campinas and Oswaldo Cruz Foundation.


Not applicable.

Author information

Authors and Affiliations



SGO:planning study, experimental design, experimental work, literature research, data analysis, manuscript preparation, manuscript editing. RRN:experimental work, data analysis. CACT:experimental work. ALMG:manuscript preparation, manuscript revision. AMRD:manuscript preparation, manuscript revision. RJ:planning study, experimental design, experimental work, literature research, data analysis, manuscript preparation, manuscript editing. FHBA: manuscript preparation, manuscript revision. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Rodrigo Jardim.

Ethics declarations

Ethics approval and consent to participate

Not applicable.

Consent for publication

Not applicable.

Competing interest

The authors declare that they have no competing interests.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Additional file 1

: S1 - Microbiome core: Shotgun and Amplicon. Text file with a list of genera belongs to the microbiome core of Shotgun metagenomics and Amplicon sequencing.

Additional file 2

: S2 - Microbiome core among Shotgun, Amplicon, and eHOMD. Text file with a list of genera belongs to the microbiome core among Shotgun metagenomics, Amplicon sequencing, and eHOMD.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Oliveira, S.G., Nishiyama, R.R., Trigo, C.A.C. et al. Core of the saliva microbiome: an analysis of the MG-RAST data. BMC Oral Health 21, 351 (2021).

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: